But let your communication be Yea, yea; Nay, nay: for whatsoever is more than these cometh of evil
Tuesday, November 27th, 2007

How to get closer to the target

Attending last week another Illumina sequencer course, I still have the question how to enrich the target sequence. A colleague calling me this morning (thanks TB!) had a pointer to a new nature methods editorial covering three different methods- a 100-mer capture probe for each exon sized segment with the need of extremely deep resequencing and two other methods using direct hybridization of segments onto commercially oligo arrays. Aren´t there any other protocols?

Related Posts: 454 or 0815 or 4911, A revival of DNA pooling, Materials and Methods, Once in a lifetime
Categories: Genetics + Biology
Keywords: , , , , , , , , ,
Trackback: http://www.wjst.de/blog/blog/2007/11/27/how-to-get-closer-to-the-target/


One Response to How to get closer to the target

  1. admin says:

    Laborwelt 2008;9:6 has the description by a Roche employee how high density capture arrays by NimbleGen were used to enrich some 6726 exon carrying fragments of 0.5-2 Mb length that covered 660 genes. More on http://www.nature.com/nmeth/journal/v4/n11/abs/nmeth1109.html and http://bioinformatics.oxfordjournals.org/cgi/content/full/22/2/134 This lead to an 400-fold enrichment of target sites.