Tag Archives: sequencing

Is buccal DNA really buccal DNA?

This is an update of the recent TCRA post herewhere I argued that TCR studies shouldn’t be done with genomic DNA from peripheral blood cells. Instead, I was arguing for buccal DNA as epithelial cells will not have undergone somatic recombination. Only last week, however, I came across an earlier letter about DNA-based assessment of chimerism after allogeneic blood stem cell transplantation (BSCT). Continue reading Is buccal DNA really buccal DNA?

3,93 mutations / Mb

1 MB is 1 Megabyte is 1,000,000 bytes and 1 Mb is 1 Megabase is 1,000,000 nucleotides. Although a new nature paper doesn’t make any fuss about it, there are 3,93 mutations / Mb in cancer tissues (in total they found 1,007 mutations by scanning 274 Mb from 210 cancer tissues). Continue reading 3,93 mutations / Mb