{"id":11190,"date":"2018-12-16T09:12:29","date_gmt":"2018-12-16T08:12:29","guid":{"rendered":"http:\/\/www.wjst.de\/blog\/?p=11190"},"modified":"2018-12-17T17:56:38","modified_gmt":"2018-12-17T16:56:38","slug":"verruckte-experimente","status":"publish","type":"post","link":"https:\/\/www.wjst.de\/blog\/sciencesurf\/2018\/12\/verruckte-experimente\/","title":{"rendered":"Verr\u00fcckte Experimente"},"content":{"rendered":"<p>Bisher dachte ich immer,\u00a0<a href=\"https:\/\/www.nature.com\/articles\/srep35363\">Optogenetik<\/a> sei die genetische Aufkl\u00e4rung von optischen Anomalien, wie Linsendeformationen oder Netzhauterkrankungen. Aber offensichtlich liege ich damit falsch, wenn ich mir die neue <a href=\"https:\/\/www.nature.com\/articles\/s41592-018-0178-9\">NM<\/a> Arbeit anschaue, in der ein Hybrid Crispr-Cas9 durch Licht angeschaltet werden kann.<\/p>\n<blockquote><p>We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent <i>Streptococcus pyogenes<\/i> Cas9 inhibitor, and the LOV2 photosensor from <i>Avena sativa<\/i>. Coexpression of these proteins with CRISPR\u2013Cas9 effectors enabled light-mediated genome and epigenome editing, and revealed rapid Cas9 genome targeting in human cells.<\/p><\/blockquote>\n<p>Die Optogenetik ist ein richtig <a href=\"https:\/\/www.scientifica.uk.com\/neurowire\/top-10-discoveries-in-10-years-of-optogenetics\">grosses Feld<\/a> mittlerweile. Es ist eine interessante Option allemal, die Enzymaktivit\u00e4t <a href=\"https:\/\/www.scientifica.uk.com\/neurowire\/top-10-discoveries-in-10-years-of-optogenetics\">intrazellul\u00e4r an- und abzuschalten zu k\u00f6nnen.<\/a><\/p>\n<p>Die <a href=\"https:\/\/www.faz.net\/aktuell\/wissen\/leben-gene\/ein-lichtschalter-fuer-die-genschere-15935276.html\">FAZ<\/a> weiss dazu aber auch ein Experiment, M\u00e4use auf Knopfdruck im Kreis laufen zu lassen, in dem man den motorischen Cortex \u00fcber Licht aktiviert. Danke an <a href=\"http:\/\/www.julietteirmer.de\">Juliette Irmer<\/a> f\u00fcr den Link!<\/p>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube.com\/embed\/88TVQZUfYGw\" width=\"560\" height=\"315\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>Was f\u00fcr Experimente und was f\u00fcr eine Hybris &#8230; Das <a href=\"https:\/\/www.wsj.com\/articles\/deformities-alarm-scientists-racing-to-rewrite-animal-dna-11544808779\">WSJ<\/a> zu einem \u00e4hnlich gelagerten Tierversuch<\/p>\n<blockquote><p>The stumbles show the risks of racing ahead with such experiments, even as many governments work to clear regulatory pathways to bring meat, eggs and dairy from gene-edited animals to store shelves. Bioethicists and many geneticists have raised doubts about applying the gene-editing technology to animals and especially humans, given the continued uncertainties in both the science and the lab and field results.<\/p><\/blockquote>\n\n<p>&nbsp;<\/p>\n<div class=\"bottom-note\">\n  <span class=\"mod1\">CC-BY-NC Science Surf , accessed 05.04.2026<\/span>\n <\/div>","protected":false},"excerpt":{"rendered":"<p>Bisher dachte ich immer,\u00a0Optogenetik sei die genetische Aufkl\u00e4rung von optischen Anomalien, wie Linsendeformationen oder Netzhauterkrankungen. Aber offensichtlich liege ich damit falsch, wenn ich mir die neue NM Arbeit anschaue, in der ein Hybrid Crispr-Cas9 durch Licht angeschaltet werden kann. We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and &hellip; <a href=\"https:\/\/www.wjst.de\/blog\/sciencesurf\/2018\/12\/verruckte-experimente\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">Verr\u00fcckte Experimente<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[3082,391],"class_list":["post-11190","post","type-post","status-publish","format-standard","hentry","category-genetics-biology","tag-hybris","tag-hybrid"],"_links":{"self":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/11190","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/comments?post=11190"}],"version-history":[{"count":9,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/11190\/revisions"}],"predecessor-version":[{"id":11201,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/11190\/revisions\/11201"}],"wp:attachment":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/media?parent=11190"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/categories?post=11190"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/tags?post=11190"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}