{"id":12504,"date":"2013-05-14T09:02:11","date_gmt":"2013-05-14T08:02:11","guid":{"rendered":"http:\/\/www.wjst.de\/blog\/?p=12504"},"modified":"2019-05-14T09:02:25","modified_gmt":"2019-05-14T08:02:25","slug":"how-much-dna-molecules-are-in-a-pcr","status":"publish","type":"post","link":"https:\/\/www.wjst.de\/blog\/sciencesurf\/2013\/05\/how-much-dna-molecules-are-in-a-pcr\/","title":{"rendered":"How much DNA molecules are in a PCR"},"content":{"rendered":"<p>Size of diploid human genome: 2 x 3 billion bp = 6 billion bp<br \/>\nAverage size of of nucleotides: \u00a0487 + 159 g\/mol = 646 g\/mol<br \/>\nSingle cell DNA amount: 6 billion * 646 g\/mol = 3.87 pg<br \/>\nIn 25 ng PCR human genomic DNA : 25000 pg \/ 3.87 pg = 6459 copies<\/p>\n\n<p>&nbsp;<\/p>\n<div class=\"bottom-note\">\n  <span class=\"mod1\">CC-BY-NC Science Surf , accessed 10.04.2026<\/span>\n <\/div>","protected":false},"excerpt":{"rendered":"<p>Size of diploid human genome: 2 x 3 billion bp = 6 billion bp Average size of of nucleotides: \u00a0487 + 159 g\/mol = 646 g\/mol Single cell DNA amount: 6 billion * 646 g\/mol = 3.87 pg In 25 ng PCR human genomic DNA : 25000 pg \/ 3.87 pg = 6459 copies &nbsp; &hellip; <a href=\"https:\/\/www.wjst.de\/blog\/sciencesurf\/2013\/05\/how-much-dna-molecules-are-in-a-pcr\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">How much DNA molecules are in a PCR<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-12504","post","type-post","status-publish","format-standard","hentry","category-genetics-biology"],"_links":{"self":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/12504","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/comments?post=12504"}],"version-history":[{"count":2,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/12504\/revisions"}],"predecessor-version":[{"id":12506,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/12504\/revisions\/12506"}],"wp:attachment":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/media?parent=12504"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/categories?post=12504"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/tags?post=12504"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}