{"id":1359,"date":"2007-11-27T20:05:13","date_gmt":"2007-11-27T18:05:13","guid":{"rendered":"http:\/\/www.wjst.de\/blog\/2007\/11\/27\/how-to-get-closer-to-the-target\/"},"modified":"2008-02-20T11:38:42","modified_gmt":"2008-02-20T09:38:42","slug":"how-to-get-closer-to-the-target","status":"publish","type":"post","link":"https:\/\/www.wjst.de\/blog\/sciencesurf\/2007\/11\/how-to-get-closer-to-the-target\/","title":{"rendered":"How to get closer to the target"},"content":{"rendered":"<p>Attending last week another Illumina sequencer course, I still have the question how to enrich the target sequence. A colleague calling me this morning (thanks TB!) had a pointer to a new <a href=\"http:\/\/www.nature.com\/nmeth\/journal\/v4\/n11\/abs\/nmeth1107-891.html\">nature methods<\/a> editorial covering three different methods-  a 100-mer capture probe for each exon sized segment with the need of extremely deep resequencing and two other methods using direct hybridization of segments onto commercially oligo arrays. Aren\u00c2\u00b4t there any other protocols? <\/p>\n\n<p>&nbsp;<\/p>\n<div class=\"bottom-note\">\n  <span class=\"mod1\">CC-BY-NC Science Surf , accessed 06.04.2026<\/span>\n <\/div>","protected":false},"excerpt":{"rendered":"<p>Attending last week another Illumina sequencer course, I still have the question how to enrich the target sequence. A colleague calling me this morning (thanks TB!) had a pointer to a new nature methods editorial covering three different methods- a 100-mer capture probe for each exon sized segment with the need of extremely deep resequencing &hellip; <a href=\"https:\/\/www.wjst.de\/blog\/sciencesurf\/2007\/11\/how-to-get-closer-to-the-target\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">How to get closer to the target<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[384,1671,1670,1669,1673,1675,1674,1672,1668,1676],"class_list":["post-1359","post","type-post","status-publish","format-standard","hentry","category-genetics-biology","tag-illumina","tag-colleague","tag-hybridization","tag-nature-methods","tag-pointer","tag-protocols","tag-segment","tag-segments","tag-target-sequence","tag-tb"],"_links":{"self":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/1359","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/comments?post=1359"}],"version-history":[{"count":0,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/1359\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/media?parent=1359"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/categories?post=1359"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/tags?post=1359"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}