{"id":1431,"date":"2008-01-07T19:20:16","date_gmt":"2008-01-07T17:20:16","guid":{"rendered":"http:\/\/www.wjst.de\/blog\/2008\/01\/07\/screening-steroid-activity\/"},"modified":"2008-01-07T19:22:40","modified_gmt":"2008-01-07T17:22:40","slug":"screening-steroid-activity","status":"publish","type":"post","link":"https:\/\/www.wjst.de\/blog\/sciencesurf\/2008\/01\/screening-steroid-activity\/","title":{"rendered":"Screening steroid activity"},"content":{"rendered":"<p>A paper in <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18172821?dopt=AbstractPlus\">J Drug Target<\/a> shows a nice property of a cell line <\/p>\n<blockquote><p>Eight repeats of the glucocorticoid response element (GRE) were cloned into [&#8230;] vector, and the resulting recombinant plasmid [&#8230;] was stably transfected into the 293E cells. The stable and sensitive cell line [&#8230;] was selected by dexamethasone (DEX) using fluorescent microscopy and fluorescence-activated cell sorting. [&#8230;] The expression of GFP4 in the cell line was under the control of GRE, up-regulated by DEX treatment and down-regulated by phorbol myristate acetate (PMA).\n<\/p><\/blockquote>\n<p>as it could be nicely used for the steroid activity of any compound.<\/p>\n\n<p>&nbsp;<\/p>\n<div class=\"bottom-note\">\n  <span class=\"mod1\">CC-BY-NC Science Surf , accessed 13.04.2026<\/span>\n <\/div>","protected":false},"excerpt":{"rendered":"<p>A paper in J Drug Target shows a nice property of a cell line Eight repeats of the glucocorticoid response element (GRE) were cloned into [&#8230;] vector, and the resulting recombinant plasmid [&#8230;] was stably transfected into the 293E cells. The stable and sensitive cell line [&#8230;] was selected by dexamethasone (DEX) using fluorescent microscopy &hellip; <a href=\"https:\/\/www.wjst.de\/blog\/sciencesurf\/2008\/01\/screening-steroid-activity\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">Screening steroid activity<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8],"tags":[],"class_list":["post-1431","post","type-post","status-publish","format-standard","hentry","category-asthma-allergy"],"_links":{"self":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/1431","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/comments?post=1431"}],"version-history":[{"count":0,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/1431\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/media?parent=1431"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/categories?post=1431"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/tags?post=1431"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}