{"id":1499,"date":"2008-03-01T13:21:33","date_gmt":"2008-03-01T11:21:33","guid":{"rendered":"http:\/\/www.wjst.de\/blog\/2008\/03\/01\/is-there-any-environmental-conditioning-of-vitamin-d-metabolism\/"},"modified":"2008-03-01T13:28:36","modified_gmt":"2008-03-01T11:28:36","slug":"is-there-any-environmental-conditioning-of-vitamin-d-metabolism","status":"publish","type":"post","link":"https:\/\/www.wjst.de\/blog\/sciencesurf\/2008\/03\/is-there-any-environmental-conditioning-of-vitamin-d-metabolism\/","title":{"rendered":"Is there any environmental conditioning of vitamin D metabolism?"},"content":{"rendered":"

So far, I haven’t seen so much work about epigenetic regulation of vitamin D. There was already a paper in 2005<\/a> that showed how treatment with the<\/p>\n

\nmethylation inhibitor 5-aza-2′-deoxycytidine together with the deacetylation inhibitor trichostatin A resulted in elevation of both CYP27B1 and CYP24 mRNA expression demonstrating that even in normal human prostate cells expression of Vitamin D hydroxylases may be under epigenetic control\n<\/p><\/blockquote>\n


\nA recent review<\/a> confirmed somewhere hidden in the text that<\/p>\n

\n… treatment with 1alpha,25(OH)2D3 induced DNA methylation at CpG sites in the promotor and exon regions of the CYP27B1 gene (data not shown).\n<\/p><\/blockquote>\n

As no details are given I am plotting here the human CYP27B1 region (chr12:56,442,385-56,447,243) with additional +1500 bp on both sides by means of the EBI server<\/a>.
\ncpgplot-20080301-103409643648051.png<\/a>
\nwhich confirms the assumed CpG islands.<\/p>\n

The situation is much clearer with an excellent paper on the rodent CYP24A1 that identified two CpG islands
\ncpgplot2.png<\/a>
\nHere is the EBI plot from the human CYP24A1 sequence
\ncpgplot-20080301-110740403510451.png<\/a>
\nUsing bisulfite sequencing, there was convincing evidence that both islands were hypermethylated in tumor derived endothelial cells; treatment with 5-aza-2′-deoxycytidine restored CYP24A1 promotor activity.
\nI do not know, if or when the human vitamin hydroxylases are being methylated but given the large variation of human 25-OH-D3 serum levels (under comparable environmental conditions) there could be an early conditioning of these enzymes.<\/p>\n\n

 <\/p>\n

\n CC-BY-NC Science Surf , accessed 09.04.2026<\/span>\n <\/div>","protected":false},"excerpt":{"rendered":"

So far, I haven’t seen so much work about epigenetic regulation of vitamin D. There was already a paper in 2005 that showed how treatment with the methylation inhibitor 5-aza-2′-deoxycytidine together with the deacetylation inhibitor trichostatin A resulted in elevation of both CYP27B1 and CYP24 mRNA expression demonstrating that even in normal human prostate cells … Continue reading Is there any environmental conditioning of vitamin D metabolism?<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[10],"tags":[1725,1287],"class_list":["post-1499","post","type-post","status-publish","format-standard","hentry","category-sunshine-vitamin","tag-epigenetic-regulation","tag-vitamin-d"],"_links":{"self":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/1499","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/comments?post=1499"}],"version-history":[{"count":0,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/1499\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/media?parent=1499"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/categories?post=1499"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/tags?post=1499"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}