{"id":15387,"date":"2020-02-19T02:46:06","date_gmt":"2020-02-19T02:46:06","guid":{"rendered":"http:\/\/www.wjst.de\/blog\/?p=15387"},"modified":"2020-02-19T15:03:14","modified_gmt":"2020-02-19T15:03:14","slug":"what-a-shit-show-life-sciences-have-at-least-partially-become","status":"publish","type":"post","link":"https:\/\/www.wjst.de\/blog\/sciencesurf\/2020\/02\/what-a-shit-show-life-sciences-have-at-least-partially-become\/","title":{"rendered":"&#8220;what a shit-show life-sciences have (at least partially) become&#8221;"},"content":{"rendered":"<p>quoting <a href=\"https:\/\/www.reddit.com\/r\/labrats\/comments\/f4tb9a\/eternal_sunshine_of_the_fraudless_blot\/\">another blog<\/a> from my current reading list<\/p>\n<blockquote><p>an application specialist &#8230;\u00a0 gave a day of training on qPCR applications in general and these instruments specifically. The scientist got to a point in her presentation where she was describing frequent errors and pitfalls in qPCR methods for quantitating gene expression, and she gave about 10 different examples that started out &#8220;I visited this one lab, and upon discussing their workflow with them it turned out they were making huge error such-and-such.&#8221; At this point, I raised my hand and asked for her honest estimation of how much confidence she places in qPCR or RT-PCR data that appear in standard journals. She paused for a few seconds and then tentatively admitted &#8220;maybe 25%.&#8221;<\/p><\/blockquote>\n\n<p>&nbsp;<\/p>\n<div class=\"bottom-note\">\n  <span class=\"mod1\">CC-BY-NC Science Surf , accessed 11.04.2026<\/span>\n <\/div>","protected":false},"excerpt":{"rendered":"<p>quoting another blog from my current reading list an application specialist &#8230;\u00a0 gave a day of training on qPCR applications in general and these instruments specifically. The scientist got to a point in her presentation where she was describing frequent errors and pitfalls in qPCR methods for quantitating gene expression, and she gave about 10 &hellip; <a href=\"https:\/\/www.wjst.de\/blog\/sciencesurf\/2020\/02\/what-a-shit-show-life-sciences-have-at-least-partially-become\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">&#8220;what a shit-show life-sciences have (at least partially) become&#8221;<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8],"tags":[],"class_list":["post-15387","post","type-post","status-publish","format-standard","hentry","category-asthma-allergy"],"_links":{"self":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/15387","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/comments?post=15387"}],"version-history":[{"count":2,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/15387\/revisions"}],"predecessor-version":[{"id":15389,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/15387\/revisions\/15389"}],"wp:attachment":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/media?parent=15387"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/categories?post=15387"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/tags?post=15387"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}