{"id":2743,"date":"2009-07-17T08:29:06","date_gmt":"2009-07-17T06:29:06","guid":{"rendered":"http:\/\/www.wjst.de\/blog\/?p=2743"},"modified":"2009-07-17T08:30:48","modified_gmt":"2009-07-17T06:30:48","slug":"dna-sudoku","status":"publish","type":"post","link":"https:\/\/www.wjst.de\/blog\/sciencesurf\/2009\/07\/dna-sudoku\/","title":{"rendered":"DNA sudoku"},"content":{"rendered":"<p>I am a <a href=\"https:\/\/www.wjst.de\/blog\/2006\/12\/22\/a-revival-of-dna-pooling\/\">great fan of DNA pooling<\/a> (mainly for cost reasons). During our recent experiments we have lost the identity of a single DNA source by pooling. Then we found that the source DNA may be tagged with a unique oligo allowing the assembler to reconstruct the DNA source from the pool. He comes another variation of <a href=\"http:\/\/www.nature.com\/nrg\/journal\/vaop\/ncurrent\/full\/nrg2646.html\">The Sequencing Game<\/a><\/p>\n<blockquote><p>\nThe pooling of DNA sequencing samples is not new, but current protocols rely on bar coding each sample with a short oligonucleotide, which is then used to associate a read to the correct sample. This approach is laborious, however, as a unique tag has to be created for each sample. The new method creates pools of samples, and then associates a bar code to each pool, rather than to each individual sequence.\n<\/p><\/blockquote>\n\n<p>&nbsp;<\/p>\n<div class=\"bottom-note\">\n  <span class=\"mod1\">CC-BY-NC Science Surf , accessed 05.04.2026<\/span>\n <\/div>","protected":false},"excerpt":{"rendered":"<p>I am a great fan of DNA pooling (mainly for cost reasons). During our recent experiments we have lost the identity of a single DNA source by pooling. Then we found that the source DNA may be tagged with a unique oligo allowing the assembler to reconstruct the DNA source from the pool. He comes &hellip; <a href=\"https:\/\/www.wjst.de\/blog\/sciencesurf\/2009\/07\/dna-sudoku\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">DNA sudoku<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[680],"class_list":["post-2743","post","type-post","status-publish","format-standard","hentry","category-genetics-biology","tag-dna-pooling"],"_links":{"self":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/2743","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/comments?post=2743"}],"version-history":[{"count":4,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/2743\/revisions"}],"predecessor-version":[{"id":2754,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/2743\/revisions\/2754"}],"wp:attachment":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/media?parent=2743"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/categories?post=2743"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/tags?post=2743"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}