{"id":81,"date":"2006-08-14T12:52:15","date_gmt":"2006-08-14T10:52:15","guid":{"rendered":"http:\/\/146.107.134.84\/wordpress\/index.php\/2006\/08\/14\/taqman%c2%ae-based-genotyping-what%c2%b4s-new\/"},"modified":"2010-12-27T17:50:22","modified_gmt":"2010-12-27T15:50:22","slug":"taqman-%c2%ae-based-genotyping-what%c2%b4s-new","status":"publish","type":"post","link":"https:\/\/www.wjst.de\/blog\/sciencesurf\/2006\/08\/taqman-%c2%ae-based-genotyping-what%c2%b4s-new\/","title":{"rendered":"Taqman \u00ae based genotyping &#8211; What\u00b4s new?"},"content":{"rendered":"<p>You may have read the contribution of Monika Werner and me in &#8220;<a href=\"http:\/\/www.amazon.de\/gp\/product\/082472884X\/028-5069077-8449355?v=glance&amp;n=52044011\">Pharmacogenetics<\/a>&#8221; about &#8220;Methods of Genotyping&#8221;.<\/p>\n<p>A point that we did not cover when describing the 5-prime-nuclease  assay is the neverending miniaturisation of assays. At a recent HUGO meeting I have already seen British <a href=\"http:\/\/www.kbioscience.co.uk\/genotyping\/index.htm\">Kbioscience<\/a> offering an extremely flat, black, ca. 8 x 12 x 0.2 cm, 1,536 well plastic plate (size recalled from memory) that is sealed after filling in primer \/ mastermix and DNA. Up to 1,000 sealed plates are then water-bathed for temperature cycling.<\/p>\n<p>A few days ago another system made it on my desk: U.S. <a href=\"http:\/\/www.biotrove.com\/products\/open_array\/snp\/\">BioTrove<\/a> sent me a demo array that is about 6,4 x 2 x 0,01 cm has precoated primer <span class=\"headersmaller\">holes for 3,072 assays where you just add your DNA + mastermix before sealing and cycling it on  a conventional  thermocycler.<\/span><\/p>\n\n<p>&nbsp;<\/p>\n<div class=\"bottom-note\">\n  <span class=\"mod1\">CC-BY-NC Science Surf , accessed 09.04.2026<\/span>\n <\/div>","protected":false},"excerpt":{"rendered":"<p>You may have read the contribution of Monika Werner and me in &#8220;Pharmacogenetics&#8221; about &#8220;Methods of Genotyping&#8221;. A point that we did not cover when describing the 5-prime-nuclease assay is the neverending miniaturisation of assays. At a recent HUGO meeting I have already seen British Kbioscience offering an extremely flat, black, ca. 8 x 12 &hellip; <a href=\"https:\/\/www.wjst.de\/blog\/sciencesurf\/2006\/08\/taqman-%c2%ae-based-genotyping-what%c2%b4s-new\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">Taqman \u00ae based genotyping &#8211; What\u00b4s new?<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-81","post","type-post","status-publish","format-standard","hentry","category-genetics-biology"],"_links":{"self":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/81","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/comments?post=81"}],"version-history":[{"count":1,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/81\/revisions"}],"predecessor-version":[{"id":5307,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/posts\/81\/revisions\/5307"}],"wp:attachment":[{"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/media?parent=81"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/categories?post=81"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.wjst.de\/blog\/wp-json\/wp\/v2\/tags?post=81"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}