Tag Archives: Alzheimer

The amyloid Western blot: Schrag vs Lesné

The amyloid analysis published in Nature has been commented at PubPeer and also earned a commentary  of Charles Piller in  Science. His “Blots on a field” news story is leading now even to an expression of concern by Nature.

The editors of Nature have been alerted to concerns regarding some of the figures in this paper. Nature is investigating these concerns, and a further editorial response will follow as soon as possible.

IMHO there are many artifacts including horizontal lines in Fig 2 when converting it to false color display. I can not attribute the lines to any splice mark  and sorry – this is a 16 year old gel image, (Basically an eternity has passed in terms of my own Nikon history with 5 generations from D2x to Z9) So don’t expect any final conclusion here as long as we cannot get the original images here.

false color display of Fig 2 a “the presence of 8 M urea did not alter the electrophoretic pattern of Aß oligomers in extracellular-enriched extracts from 12- to 20-month-oldbrains ofTg2576þ/2mice that were probed with 6E10 antibodies.”

Continue reading The amyloid Western blot: Schrag vs Lesné